The adsorption of protein on poly dimethylsiloxane(PDMS) which often used to make microchip was studied in this paper.Effects of the pH and concentration of buffer solution,three surfactant concentration,and time on the adsorption of bovine serum albumin(BSA) were investigated in detail.The absorbency of BSA was measured with the ultraviolet spectrophotometer.The results showed that the adsorption of BSA reached minimum when buffer solution pH was 7.0 and concentration was 40 mmol/L.The suppression of protein adsorption was best when concentration of surfactant Brij35,sodium dodecalkyl sulfate(SDS),and methyl cellulose(MC) was 0.04%,0.04%,and 0.06%,respectively.The absorbency of BSA showed fluctuation with time.All results would be useful to the surface modification of PDMS microchip and the reducing of protein adsorption.
利用乙二醇乙醚和精制大豆油合成出一种新型生物柴油——乙二醇乙醚豆油单酯。通过正交试验探讨最佳合成条件,对产物进行了红外光谱分析和氢核磁共振波谱分析,并利用单缸机研究柴油机的炭烟排放情况。结果表明,这种新型生物柴油的最佳合成条件为醇油摩尔比6∶1,催化剂用量0.5%,反应温度90℃,反应时间30 m in;燃烧新型生物柴油后,柴油机的炭烟排放水平大幅度降低。
