The cardiac and vascular effect of ethanolamine nitrate ester ferulate (ENF) wasinvestigated on isolated working guinea pig heart (IWH) and rat aortic ring. The coronary blood flowwas remarkably increased while the cardiac functions of IWH were not significantly altered by ENFranging from 10-8 to 10-4 mol/L. The percentages of vasorelaxation of ENF t 10 μmol/L) and nicoran-dil (NIC, 10 μmol/L) on K+ (80 mmol/L)-induced contraction in aortic rings were 72 ± 8% and17±8%, respectively. The relaxation effects of ENF and NIC on phenylephrine (PE, 5 μmol/L) in-duced contraction were in a dose-dependent manner. When vessel segments were exposed to PE withmethylene blue (MB, 10 μmol/L) or with glibenclamide (GLI, 10 pmol/L), the percentages of relaxa-tion of ENF (10 μmol/L) and NIC ( 1 0 μmol/L) were 4 1 ±11 % and 49±7% or 76± 14% and the 33±9%,respectively. The results suggested that ENF improved coronary circulation and the vasodilation of ENF was mediated bv nitrate-like action.
目的:研究PCO—Pin,Nic,Lem及RP对VMSC内[Ca^(2+)]_i的改变及其可能机制。方法:VSMC加入Fura-2 AM 2.5μmol·L^(-1)37℃下孵育50min,[Ca^(2+)]_i用荧光分光光度计检测。结果:4种PCO能较弱地抑制K^+30 mmol·L^(-1)诱导的[Ca^(2+)]_i增加,但明显抑制ATP 0.1mmol·L^(-1)诱导的[Ca^(2+)]_i峰相及持续相增加,且呈剂量依赖性。格列苯脲完全阻断Pin,Lem及RP的作用,只部分抑制Nic的作用。无钙液中先给4种PCO,能显著抑制ATP诱导的[Ca^(2+)]_i峰相增加。结论:4种PCO均抑制ATP诱导的[Ca^(2+)]_i增加,此作用与减少细胞外钙内流及细胞内钙释放有关。
